Downregulation of thromboxane A2 and angiotensin II type 1 receptors associated with aging-related decrease in internal anal sphincter tone.

Aging-associated decrease in internal anal sphincter (IAS) tone (AADI) is a major contributor in the rectoanal incontinence (RI). To determine the pathogenesis of AADI, we investigated the effect of aging on GPCR activation and related downstream signaling. We particularly investigated two GPCRs that characterize IAS smooth muscle cells (SMCs): thromboxane A 2 and angiotensin II type 1. Two groups of Fischer 344 rats (6-month-old [young group] and 26-month-old [old group]) were employed to determine the GPCR function by isometric contraction, the expressions of GPCRs, and their downstream regulatory signaling proteins (regulator of G-protein signaling 2, RGS2; GPCR Kinase 5, GRK5; and β-arrestin, Arrb2) using RT-PCR, qPCR, and western blot analyses. We used reversible biotinylation to monitor the GPCR trafficking using SMCs. Aging selectively attenuated thromboxane A 2 and Ang II-induced IAS contraction. RT-PCR, qPCR, and WB data revealed a significant decrease in the expressions of the GPCRs and increase in the expression of RGS2, GRK5, and Arrb2. The increased GPCR internalization and decreased recycling under aging were validated by reversible biotinylation. We conclude that downregulation of GPCR, accompanied by upregulation of regulatory proteins, plays an important role in receptor desensitization and may be important underlying mechanisms of RI in certain aging patients

Mathematical transforms and image compression: A review

It is well known that images, often used in a variety of computer and other scientific and engineering applications, are difficult to store and transmit due to their sizes. One possible solution to overcome this problem is to use an efficient digital image compression technique where an image is viewed as a matrix and then the operations are performed on the matrix. All the contemporary digital image compression systems use various mathematical transforms for compression. The compression performance is closely related to the performance by these mathematical transforms in terms of energy compaction and spatial frequency isolation by exploiting inter-pixel redundancies present in the image data. Through this paper, a comprehensive literature survey has been carried out and the pros and cons of various transform-based image compression models have also been discussed

COMPARATIVE STUDY OF MARKETING STRATEGY OFPATANJALI AND P AND G

To the development of effective marketing strategy organization need to break down its various marketing function according to current situation of market, this process helps marketing managers to be in strong position to understand. Hence marketing strategy involves developing a company vision and implementing various policies that will enables organization to maintain its vision. The present research strategy dealt with marketing strategy of Patanjali and Procter and Gamble with respect to fast moving consumer goods, in this process research study conducted comparative analysis of marketing strategy between both selected companies. The whole research work is based on various dimensions of marketing strategy from the customer point of view, these dimensions are brand, marketing, marketing channel, marketing tactics and promotional schemes

Understanding the chapatti making attributes of the Indian wheats – II: The rheological basis

The concept of good chapati varies from individual to individual and depends mainly on the rheological properties of the dough used to prepare the chapaties. The research project was largely built around two type of plant meterials, the tall traditional cultivars of the pre-dwarfing era, and three backcross recombinant populations (BC1F5 generation) C 273/PBW 343//PBW 343 (70 lines), C 306/PBW 534//PBW 534 (70 lines) and C 518/PBW 343//PBW 343 (80 lines). Association of traits studied with chapati score in set of cultivars and genetic stocks Starch pasting characteristics showed mild negative correlation with chapati quality, which is again contrasting, to the requirements of bread making. The correlations were not consistent over years possibly due to environmental factor (temperature, rainfall, fertilizer and irrigations etc.) and due to change in the constitution of the set. Similarly mixographic traits showed negative association chapati making quality. The correlations which prevail in the populations carry much greater weight as these have persisted over several rounds of recombination and are likely to reflect under lying causes of superior chapati quality. As various components of chapati quality would be disassembled, the relative levels of correlations for individual traits would be uncovered. Among the starch pasting characteristics, final viscosity and setback were consistently negatively associated with the chapati making quality. In case of the mixographic traits, mixing tolerance index is negatively associated whereas rate of dough development has consistent positive correlation with chapati quality

Understanding the chapati making attributes of the Indian wheats – I: The physico-chemical basis

The research project was largely built around the tall traditional cultivars of the pre-dwarfing era, which were known to excel for chapati quality. These included C 306, C 518 and C 273. The few cultivars that had emanated from crosses of these superior chapati quality wheats with dwarf wheats formed another important component of this set and included WG 357, PBW 175, PBW 154, PBW 226, Lok 1 among others. Asecond set of materials, three backcross recombinant populations (BC1F5 generation) derived from C 273/PBW 343//PBW 343 (70 lines), C 306/PBW 534//PBW 534 (70 lines) and C 518/PBW 343//PBW 343 (80 lines) were also studied to arrive at some conclusion. Various physico-chemical characters(Grain appearance score,Grain hardness,Test weight,1000-grain weight,Yellow berry,Moisture content,Protein content,Gluten content, Gluten index, Sedimentation value, Phenol Test, Carotenoids, Sugar content, Diastatic activity, Falling Number) and chapati-makingscores were evaluated. Grain hardness seems to have a clear role in chapati quality with a correlation coefficient of 0.34, 0.35 and 0.17 observed in different recombinant populations.More consistent correlation was found for grain appearance ranging from 0.26 to 0.36 in the populations.Consistent high positive correlations have showed up for diastase activity, which ranged from 0.32 to 0.46.This consistent behaviour is a strong evidence for the role of this trait in chapati making quality.Diastase activity emerges as a more consistent and stronger contributor to chapati making quality. Phenol score may not serve as a suitable indicator of chapati quality

Understanding the chapati making attributes of the Indian wheats – I: The physico-chemical basis

The research project was largely built around the tall traditional cultivars of the pre-dwarfing era, which were known to excel for chapati quality. These included C 306, C 518 and C 273. The few cultivars that had emanated from crosses of these superior chapati quality wheats with dwarf wheats formed another important component of this set and included WG 357, PBW 175, PBW 154, PBW 226, Lok 1 among others. Asecond set of materials, three backcross recombinant populations (BC1F5 generation) derived from C 273/PBW 343//PBW 343 (70 lines), C 306/PBW 534//PBW 534 (70 lines) and C 518/PBW 343//PBW 343 (80 lines) were also studied to arrive at some conclusion. Various physico-chemical characters(Grain appearance score,Grain hardness,Test weight,1000-grain weight,Yellow berry,Moisture content,Protein content,Gluten content, Gluten index, Sedimentation value, Phenol Test, Carotenoids, Sugar content, Diastatic activity, Falling Number) and chapati-makingscores were evaluated. Grain hardness seems to have a clear role in chapati quality with a correlation coefficient of 0.34, 0.35 and 0.17 observed in different recombinant populations.More consistent correlation was found for grain appearance ranging from 0.26 to 0.36 in the populations.Consistent high positive correlations have showed up for diastase activity, which ranged from 0.32 to 0.46.This consistent behaviour is a strong evidence for the role of this trait in chapati making quality.Diastase activity emerges as a more consistent and stronger contributor to chapati making quality. Phenol score may not serve as a suitable indicator of chapati quality

RhoA/ROCK Pathway is the Major Molecular Determinant of Basal Tone in Intact Human Internal Anal Sphincter

Poster presented at: Digestive Disease Week (DDW) International meeting in San Diego, California. Background and Aims Knowledge of molecular control mechanisms underlying the basal tone in the intact human IAS is critical for the pathophysiology and rational therapy for debilitating rectoanal motility disorders